Dilution & Rinse Buffers General Reagents & Lab Buffers PBS & Salines LISS
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FOR ROMANOWSKY STAINS |
Rowmanowsky stains (Giemsa, Leishmans, Wrights etc) are pH sensitive. Tinctorial (stain picture) quality can be altered by changes in the final pH of staining reagent, this can be caused by differences in the quality or source of the water used to dilute such stains. Tinctorial quality and consistency can be maintained by the use of dilution buffers, which when added to the diluent water will produce stain reagents of the required pH.
Acid pH (<7.0) will emphasize eosinophilic staining.
Basic pH (>7.0) will emphasize azureophilic (basophilic) staining.
In general, blood stains are diluted to produce solutions of slightly acid pH, 6.50-6.80.
We offer a range of buffer concentrates covering pH's 6.50 / 6.80 / 7.00 / 7.20 / 7.40 / 7.60, supplied in 10 vial or 25 vial packs each vial makes 5 litres of dilution buffer. They offer great value for money and the certainty of preparing blood stains of the required pH time and again.
To maintain the tinctorial qualities of the final stain slides should be rinsed in the same buffer.
Concentrates - ready-to-use.
High quality, accurate, time saving and good value for money.
Click Product Number for pack size / pricing / and other information.
B020 Buffer Concentrate - Sorensens phosphate buffer pH 6.5
(Each vial makes 5 litres buffer)
B010 Buffer Concentrate - Sorensens phosphate buffer pH 6.8
(Each vial makes 5 litres buffer)
B030 Buffer Concentrate - Sorensens phosphate buffer pH 7.0
(Each vial makes 5 litres buffer)
B040 Buffer Conecntrate - Sorensens phosphate buffer pH 7.2
(Each vial makes 5 litres buffer)
B050 Buffer Concentrate - Sorensens phosphate
buffer pH 7.4 
(Each vial makes 5 litres buffer)
B060 Buffer Concentrate - Sorensens phosphate buffer pH 7.6
(Each vial makes 5 litres buffer)
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B160 Drabkins Reagent, Neutral (Concentrate)
Cyanmethaemaglobin method
(each vial makes 1 litre of raedy to use reagent)
Modern buffered (neutral) formulation containing surfactants .
Determination of total haemoglobin in blood.
B180 Imidazole buffered saline
(pH 7.2 sterile ready for use)
Coagulation factor assay reagent
B190 Owren's Buffer pH 7.35
Coagulation factor assay reagent
(ready for use)
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B070 Buffer Concentrate - Iso-osmotic PBS pH 7.2
(Iso-osmotic phosphate buffered saline. 25ml makes 250ml of 1% iso-osmotic saline)
B080 Buffer Ready-to-Use . Iso-osmotic PBS pH 7.2
(As B070 but ready to use solution)
B090 PBS Concentrate pH 7.2
(Phosphate buffered saline concentrate. 100ml makes 2.5lt buffered saline)
B100 PBS Concentrate pH 7.2
(Phosphate buffered saline concentrate. 500ml makes 10lts buffered saline)
B110 PBS Concentrate pH 7.6
(Phosphate buffered saline concentrate. 100ml makes 2.5lt buffered saline)
B120 PBS Concentrate pH 7.6
(Phosphate buffered saline concentrate. 500ml makes 10lts buffered saline)
B150 Saline Concentrate
(500ml makes 10lts 0.154M Saline solution)
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B130 Saline - Low ionic stregnth (LISS)
(1 bottle makes 1 litre LISS)
B135 LISS powder
(1 vial makes 1 litre LISS)
B140 LISS ready to use solution
(4 x 250ml bottles per pack)