SP300 DIFF-3
Kit
1 kit
Stain kits Ready-to-use Stains Dilution & Rinse Buffers PBS Specialised Reagents & Buffers LISS
Mountants Fixatives Immersion oils Laboratory Solvents Dyes & Stains
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BLOOD STAINS |
Since the time when Romanowsky discovered that a decomposing beaker of aqueous Methylene blue on a window sill produced brilliant results as a component of a blood stain, very little has changed. This basic fundamental is still in play today and in combination with Eosin provides the routine technique for differentiating blood cells in every Haematology lab in the World.
We are one of the few companies in the World who make our own Romanowsky compound. We have our own technique developed and refined over almost 20 years. Our process produces a high quality, reliable product batch after batch.
This material is used to prepare the traditional blood stains. However as demands have tightened we also offer a blood stain using highly purified standardised compounds for the more critical applications.
Blood stain stability
Some Romanowsky type blood stains are inherently unstable . Although we use stabilising techniques in formulation these products still have relatively short shelf-lives. To ensure the best possible product our blood stains are produced fresh at the time of supply. This ensures highest quality at time of receipt by the customer - we also advise customers to order small amounts regularly therefore keeping their own stocks fresh.
We welcome your comments and enquiries.
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SP300 DIFF-3
Kit
1 kit
This is one of the most popular products. Used daily for routine
blood cell differentiation. It produces a stain picture similar to
that of Leishmans/Wrights stain only in a fraction of the time
[less than 1 minute]
Sample available on request
There is a 6 page technical leaflet with the kit guiding the user to
the various uses of DIFF-3
some of which are:
-- Rapid Blood Cell differentiation
-- Spermatozoa demonstration
-- Parasite demonstration
-- H.Pylori in tissue sections.
H.Pylori Blood smear [ Diff-3]
SP100 ROMANOWSKY STAIN
PACK 1 kit
Prepared using highly purified Romanowsky dyes.
Suitable for refilling Hematek 1 and H1000 slide staining
machines.
Contents: Blood stain 250ml
Diluent buffer 500ml
Rinse reagent 1000ml
Thin blood smear SP100
SP310 HEMA-STAR
1 Kit
The product guarantees exceptional performance every time.
It is a one-step rapid blood stain formulated from the most
pure components available combined with a buffer system
the encourages rapid staining while providing optimal pH
conditions upon dilution. Just apply stain and rinse.
Brilliant results are obtained in 1-2 minutes.
Sample and technical literature on request.
SP315 HEMA-STAR Concentrate Kit
1 kit
Exactly the same performance as HEMA-STAR . This kit
provides the reagents seperately in concentrate form.
Therefore allowing reagent to be made up fresh each time
which is a certain way to guarantee unbeatable stain
stain performance each time. All rapid blood stains have
short shelf lives. This kit with separate components has
exceptionally long life with brilliant performance
guaranteed to the last drop.
Technical leaflet available on request.
SP350 RETICULOCYTE STAIN 1 kit
In the past certain stains have proved unreliable on a batch by
batch basis for demonstrating reticulocytes. Our formulation uses
purfied components in a buffered solvent system that can be
controlled for quality far more reliable than earlier techniques.
The method involves a short incubation period after which
reticulo-filamentious material will stain dark blue, clearly defined
against a pale greenish background.
This is a high quality guaranteed performance product.
Please click the HOTSPOT in the picture above to see method
and details.
SP370 FETAL HAEMOGLOBIN
KIT 1 kit
To demonstrate HbF in blood smears.
Strict quality monitoring using HbA/HbF controls during preparation
guarantees well differentiated results.
Results obtained: HbF containing erythrocytes - Bright Deep Pink
HbA containing erythrocytes - Grey/pink outer
membrane of the 'Ghost' cells clearly defined.
Leukocytes - Blue nuclei.
SP380 IRON STAIN KIT 1 kit
Based on a reliable and well established technique to demonstrate
bound iron in erythrocytes, tissue and bone marrow.
[Prussian Blue Reaction (Pearls Stain)]
SP360 NBT REDUCTION KIT 1 kit
Heparin blood incubated with the buffered NBT Reagent.
On completion neutrophils are examined for sites of enzyme
activity demonstrated as purple NBT-Formazin deposits.
Comprehensive method sheet provided with the kit covering
stimulated and unstimulated tests.
S0355 FIELDS STAIN TWIN-PACK 1 kit
2 x 1lt Prefiltered reagent set to demonstrate P.Malariae etc in
blood by the method of J.W.Field.
Long established and reliable.
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S0390 Giemsa stain traditional
Dilute 1:9 with buffer for use. [Buffers]. Traditional Giemsa results
S0580 Jenners stain
Use in Jenner - Giemsa technique. Results same as May-Grunwalds.
S0600 Leishmans stain
Traditional Romanowsky results obtained.
Dilute 1:2 with buffer for use Should be purchased fresh for best results. Stain time 4-8 minutes.
S0690 May Grunwalds stain
Use as first stain stage in May-Grunwalds Giemsa technique.
S1370 Wrights stain
Traditional Romanowsky results obtained. Dilute 1:2 with buffer for use.
Should be purchased fresh for best results. Stain time 4-8 minutes.
S0110 Brilliant cresyl blue stain reagent
Alcoholic reagent for demonstrating reticulated cells and platelets.
Method details available on request. For guaranteed reliability we recommend SP350
S0120 Brilliant cresyl blue stain reagent
Incubation method for demonstrating reticulated cells and platelets.
Method details available on request.
S0980 New Methylene blue reagent
This is the best stain for reticulocyte staining.
Product No: SP350 (above) is the recommended product for reticulocyte demonstration and counting.
It is subject to strict quality controls that guarantees its suitability.
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FOR ROMANOWSKY STAINS |
Rowmanowsky stains (Giemsa, Leishmans, Wrights etc) are pH sensitive. Tinctorial (stain picture) quality can be altered by changes in the final pH of staining reagent, this can be caused by differences in the quality or source of the water used to dilute such stains. Tinctorial quality and consistency can be maintained by the use of dilution buffers, which when added to the diluent water will produce stain reagents of the required pH.
Acid pH (<7.0) will emphasize eosinophilic staining.
Basic pH (>7.0) will emphasize azureophilic (basophilic) staining.
In general, blood stains are diluted to produce solutions of slightly acid pH, 6.50-6.80.
We offer a range of buffer concentrates covering pH's 6.50 / 6.80 / 7.00 / 7.20 / 7.40 / 7.60, supplied in 10 vial or 25 vial packs each vial makes 5 litres of dilution buffer. They offer great value for money and the certainty of preparing blood stains of the required pH time and again.
To maintain the tinctorial qualities of the final stain slides should be rinsed in the same buffer.
Concentrates - ready-to-use.
High quality, accurate, time saving and good value for money.
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Stain Dilution Buffers
B020 Buffer Concentrate - Sorensens phosphate buffer pH 6.5
(Each vial makes 5 litres buffer)
B010 Buffer Concentrate - Sorensens phosphate buffer pH 6.8
(Each vial makes 5 litres buffer)
B030 Buffer Concentrate - Sorensens phosphate buffer pH 7.0
(Each vial makes 5 litres buffer)
B040 Buffer Conecntrate - Sorensens phosphate buffer pH 7.2
(Each vial makes 5 litres buffer)
B050 Buffer Concentrate - Sorensens phosphate
buffer pH 7.4 
(Each vial makes 5 litres buffer)
B060 Buffer Concentrate - Sorensens phosphate buffer pH 7.6
(Each vial makes 5 litres buffer)
Haematology Laboratory Buffers
B200 Barbitone buffer pH7.40
Stock buffer solution for preparing Barbitone buffered Salines.
B210 Barbitone buffered Saline pH 7.40 [Conc]
100ml concentrate makes 1 litre of ready to use reagent.
B220 Barbitone buffered Saline pH 9.50 [Conc]
100ml concentrate makes 1 litre ready to use reagent.
B070 Buffer Concentrate - Iso-osmotic PBS pH 7.2
(Iso-osmotic phosphate buffered saline. 25ml makes 250ml of 1% iso-osmotic saline)
B080 Buffer Ready-to-Use . Iso-osmotic PBS pH 7.2
(As B070 but ready to use solution)
(Phosphate buffered saline concentrate. 100ml makes 2.5lt buffered saline)
B100 PBS Concentrate pH 7.2
(Phosphate buffered saline concentrate. 500ml makes 10lts buffered saline)
B110 PBS Concentrate pH 7.6
(Phosphate buffered saline concentrate. 100ml makes 2.5lt buffered saline)
B120 PBS Concentrate pH 7.6
(Phosphate buffered saline concentrate. 500ml makes 10lts buffered saline)
B150 Saline Concentrate
(500ml makes 10lts 0.154M Saline solution)
B180 Imidazole buffered saline
(pH 7.2 sterile ready for use)
Coagulation factor assay reagent
B190 Owren's Buffer pH 7.35
Coagulation factor assay reagent
(ready for use)
Low Ionic Stregnth Saline [LISS]
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B130 Saline - Low ionic stregnth (LISS)
Concentrate form (1 bottle makes 1 litre LISS).
B135 LISS powder
Dry compound form for long shelf life. (1 vial makes 1 litre LISS)
B140 LISS ready to use solution
Sterile (4 x 250ml bottles per pack).
Haematology Laboratory Reagents
B160 Drabkins Reagent, Neutral (Concentrate)
Cyanmathaemaglobin method
(each vial makes 1 litre of reagent, supplied in a 6 vial pack)
Modern buffered (neutral) formulation containing surfactants .
Determination of total haemoglobin in blood.
SP365 Sickle Cell Tube-Test Kit 1 kit
Saponin-phosphate buffer and dithionite reagent set to demonstrate insolubility
of HbS by the tube-test (turbidity) method. 100 tests per kit.
R050 Platelet dilution fluid
Oxalate based diluting fluid for Platelet Counts.
Ready to use, ultrafiltered and sterile.
R070 RBC dilution fluid
Formal-citrate based dilution fluid for red blood cell counts.
Maintains disc-like appearance of cell without agglutination.
R090 WBC dilution fluid
Acetic-gentian based dilution fluid for leukocyte counts.
R195 Erhlich's Reagent
R220 Fouchet's Reagent
R225 Saponin
Pure white/off white saponin for sickle-cell buffer prep.
R370 Sodium chloride
Pre-weighed vials of 45 gms. To prepare 5 litres saline 0.9%.
R380 tri-Sodium citrate
Pre-weighed vials of 3.13 gms.
R390 tri-Sodium citrate
Pre-weighed vials of 3.18 gms.
Electrophoresis buffers
B200 Tris-acetate-EDTA pH 7.40
100ml concentrate makes 1 litre buffer
B210 Tris-borate-EDTA pH 8.60
Discontinous for starch gel electrophoresis of Haemoglobin, 100ml concentrate
makes 1 litre buffer.
B220 Tris-borate-EDTA pH 8.90]
Continuous for Cellulose Acetate Electrophoresis of Haemoglobin, (HbA2) ,
100ml concentrate makes 1 litre buffer.
B230 Tris-glycerol-EDTA ph 9.20
(For Hb Electrophoresis, 100ml makes 1 litre)
B240 Phosphate pH 7.90
(Webber & Osdorn, 100ml makes 1 litre)
B250 Barbitone pH 8.60
(Ionic strength 0.05, 200ml makes 1 litre)
B260 Barbitone pH 8.60
(Ionic strength 0.02, 100ml makes 1 litre)
B270 Barbitone-acetate pH 8.20
(Ionic strength 0.1, 100ml makes 1 litre)
B280 Barbitone - calcium lactate
(To make 1 litre)
B290 Barbitone-Tris-calcium lactate
(From Svendsen, to make 1 litre)
B300 Barbitone-Tricine-Tris-Calcium lactate
(From Monthony, to make 1 litre)
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M050 Aqueous Mountant
Polymer based mountant for everyday use. Trial sample available on request.
M010 Apathays Mountant
Traditional Apathays medium
M140 DPX Mountant
Synthetic polystyrene based mountant.
M170 Farrants Mounting medium
S0590 Lactophenol
M400 UV Free Aqueous Mountant
Trial sample available on request
M410 UV Free Mountant [ Xylene based]
Trial sample available on request. Supplied in glass scew top bottles.
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M180 NBF 10
10% Neutral Buffered Formalin.
M200 Formal-Saline
10% Formaldehyde-saline fixative
09190 Methanol anhydrous
Fixative quality
09290 Ethanol 99% denatured
For other Ethanol grades see Solvents
Click Product Number for pack size / pricing / and other information.
Unless specified all the oils are supplied in dispenser nozzle bottles as shown in 60 / 125 / 250ml sizes.
M245 Immersion oil
General purpose (low RI). RI: 1.14-1.15
M250 Immersion oil [standard
use]
General purpose. RI: 1.50 - 1.51
Recommended for everyday use with immersion lenses.
M255 Immersion oil [critical]
Particulate free, guaranteed RI: 1.517 - 1.518
M252 Immersion oil HV
High viscosity oil for specialised applications.
RI: 1.515 . Trial sample available on request.
M260 Cedarwood oil
Suitable for use as an immersion oil. Screw top glass bottle.
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BIOLOGICAL DYES |
We offer 250 biological dyes and stains in pure or compound form.
All of our dyes are subject to a series of chemical and clinical
controls to ensure their absolute suitability for their most common
intended uses.
A comprehensive list can be found at Dyes and Stains